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. 2014 Jul 11;142(4):603–613. doi: 10.1111/imm.12270

Figure 3.

Figure 3

Activation-induced cell death (AICD) of CD8+ T cells in c-Jun N-terminal kinase (JNK) -deficient mice after acute ectromelia virus (ECTV) infection. JNK1−/−, JNK2−/− and wild-type (WT) mice were infected subcutaneously (s.c.) with ECTV. (a) On day 8 post-infection (p.i.), spleens were harvested from groups of five mice for each strain. Lymphocytes were isolated and cultured with either PBS (D8) or CD3 plus CD28 (D8 + Ab) for 24 hr, and then stained with CD8, CD44 and Annexin V. (b) Spleen cells from WT mice in (a) were pre-incubated with SP600125 for 45 min, then cultured with CD3 plus CD28 for 24 hr and stained with CD8, CD44 and Annexin V. The dot plots are gated on CD8+ T cells, and the numbers are the percentages among CD44+ cells. Data represent average values of each group and are representative of three independent experiments. (c) Summary histograms of AICD of CD8+ T cells. Results are expressed as mean ± SEM. **P < 0·01 versus WT in D8 + Ab group, Mann–Whitney U-test.