Figure 2.
In vitro antibody production from spleen and bone marrow cells from ROSA yellow fluorescent protein transgenic (YFP Tg) mice. Splenocytes or bone marrow cells (5 × 105 cells/well in 96-well plates) at day 9 post prime or boost immunization (n = 3) were subjected to ex vivo cultures for 24 hr in influenza virus-like particle (VLP) -coated 96-well culture plates. A/PR8 VLP-specific IgG antibodies were measured by ELISA and total concentrations of IgG antibodies for each naive, prime, and boost immunized group from spleen or bone marrow were calculated accordingly. A representative is shown out of two independent experiments. *P < 0·05; ***P < 0·0001.