Figure 3.
Endogenous yellow fluorescent protein-positive (YFP+) populations in spleens and bone marrow before and after immunization. Splenocytes and bone marrow cells were harvested 9 days after prime and boost immunization. The cells were stained with surface marker antibodies and analysed by flow cytometry. YFP+ B220+ (a) and YFP+ B220− (b) cell population in naive, primed, or boosted ROSA transgenic mice. A representative is shown out of two independent experiments.