Figure 4.

Flow cytometry profile of B220 cells in CD43⁺ and CD43⁻ population. Enriched CD43⁺ (eCD43⁺ B220⁻) or CD43⁻ (eCD43⁻ B220⁺) fractionated cells were isolated from splenocytes by MACS and stained with fluorescence-conjugated B220 antibody. B220⁺ or B220⁻ cell populations were determined after gating CD43⁺ or CD43⁻ cells. The representative cell populations are shown out of three independent experiments.