Figure 4.

CD13 expression in monocytic cell line drives myeloid cell infiltration into the peritoneal cavity. Adoptive transfer of differentially labelled murine monocytic WEHI78/24 cells infected with either (a) mouse CD13 (mCD13-red) and empty vector control (EV-green) or (b) untreated mouse CD13 (mCD13-red) and SL-13 blocking antibody-treated mouse CD13 (SL13 tx mCD13-green) into thioglycollate-administered CD13 wild-type (CD13^WT) mice. (a, b) Preferential trafficking of cells expressing CD13 to the peritoneum was analysed by flow cytometry. (c) Percentage of cells present in the peripheral blood of unchallenged CD13^WT animals. Data represent average of three independent experiments. Error bars represent mean ± SEM for CD13^WT (n = 5) per mice group; **P < 0·01, ***P < 0·001.