Figure 8.

CD13 expression on both monocyte and the endothelium is essential for optimal cell trafficking in vivo. A mixture of equal number of CD13 wild-type (CD13^WT) and CD13 knockout (CD13^KO) splenic myeloid cells differentially labelled with green and red dye respectively were adoptively transferred to thioglycollate-administered CD13^WT and CD13^KO recipients. After 48 hr, peritoneal exudates from recipients of both genotypes were isolated, cytospun and analysed by microscopy (a) and flow cytometry (b). (a) Total number of green-WT or red-KO cells was calculated in the recipient mice (n = 5/genotype and 30 non-overlapping fields per animal were calculated). (b) Flow cytometric analysis of percentage of infiltrating green-WT and red-KO cells in the peritoneal lavage of CD13^WT and CD13^KO recipients (n = 3/mice per group) Data represent average of three independent experiments. Error bars represent mean ± SEM; *P < 0.05, **P < 0·01.