Table 1.

list of primers were used for regular PCR and real time –PCR

Variables	Forward primer(5-3)	Reverse primer(3-5)	Annealing temperature(C)	Product size(bp)
TLR1	GGGTCAGCTGGACTTCAGA	AAAATCCAAATGCAGGAACG	63	250
TLR2	TCGGAGTTCTCCCAGTTCTCT	TCCAGTGCTTCAACCCACAA	60	175
TLR4	TGATGTCTGCCTCGCGCCTG	AACCACCTCCACGCAGGGCT	60	98
TLR5	CACCAAACCAGGGATGCTAT	CCTGTGTATTGATGGGCAAA	60	111
TLR6	GCCACCATGCTGGTGTTGGCT	CGCCGAGTCTGGGTCCACTG	60	101
COX2	CAGCCATACAGCAAATCCT	TCTCCATAGAATCCTGTCCG	60	113
β-actin	CAAGATCATTGCTCCTCCTG	ATCCACATCTGCTGGAAGG	60	90

Sequence of TLRs, COX2 and β-actin primers used in the current investigation in RT-PCR. TLR; Toll-like receptor and COX; Cyclooxygenase.