Figure 2.

Spinal injection of CBX and Cx43 mimetic peptides 21 days after nerve injury reduces CCI-induced mechanical allodynia in the late phase. (A) Intrathecal injection of CBX (0.5 or 5 µg) rapidly (<0.5 h) and completely reversed mechanical allodynia for 5 h, in a dose-dependent manner. This inhibitory effect recovered after 24 h. (B) Intrathecal injection of Cx43 mimetic peptides (⁴³Gap26, or ^37,43Gap27) also reduced mechanical allodynia for 3 h. However, the scrambled peptide (Gap27 scrambled) had no effect. All data are mean ± SEM. The differences between groups were analysed by ANOVA. *P < 0.05, compared with vehicle, n = 6–7 mice/group.