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. 2014 Jul 21;206(2):217–230. doi: 10.1083/jcb.201401002

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© 2014 Huang et al.

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Figure 6. — AGO3 is associated with Armi at mitochondria. (A and B) w¹¹¹⁸ ovary was stained with anti-Armi (red) and anti-Tom20 (green). A shows the staining in germarium and early egg chambers. B shows the staining in mid-to-late-stage egg chamber. The mitochondrion localization of Armi with Tom20 is indicated by arrows. (C and D) Ovary expressing Zuc-GFP driven by nosP-gal4:vp16 was stained for Armi (red) and GFP (green). C shows their staining in germarium and early egg chambers. D shows the staining in mid-to-late-stage egg chamber. The mitochondrion localization of Armi with Zuc-GFP is indicated by arrows. Bars, 10 µm. (E) Subcellular fractionation of mitochondria from ovaries. Ovaries were homogenized in a homogenization buffer and then subjected to differential centrifugation and sucrose gradient centrifugation as shown in the diagram. (F) Western blot assays were performed to measure the abundance of Armi, and specific markers in the purified mitochondrion and cytosol fractions of wild-type ovaries. In this assay, the α-tubulin and GAPDH were used as cytosol-specific markers and Tom20 and cytochrome c as mitochondrion-specific markers. (G) Western blot assays were performed to show the abundances of Armi, GFP, Tom20, and α-tubulin in the indicated fractions from ovary expressing Zuc-GFP driven by nosP-gal4:vp16.