Figure 1.

Diagram depicting the genomic region, deletion and complementation fragments examined in this study. The genomic region contains nine genes indicated with arrows: (1) MMSYN1_0839, (2) nusG, and (3–9) MMSYN1_0841- MMSYN1_0847. The orientation of each gene is indicated (right-pointing arrow denotes a clock-wise direction in the genome). The deletion removes seven genes shown in green (genes 2–8), starting with the initiator codon of gene 2 and ending with the stop codon of gene 8. Complementation fragment 1 includes gene 1, gene 2 and their corresponding upstream and downstream intergenic regions. This complementation fragment was used in a marker-less approach (see Figure 2). Complementation fragment 2 includes only the ORF of gene 2. This fragment was combined with an expression vector and used in a “marker-driven” approach.