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. 2014 Jul 23;5:369. doi: 10.3389/fmicb.2014.00369

Figure 6.

Figure 6

Comparison of a rescued JCVI-syn1.0 ΔL clone with the positive- and negative-control strains for a clone (ML1) obtained using the marker-less approach (A,B) and for a clone (MD1) obtained using the marker-driven approach (C,D). (B) and (D) are magnified views of colonies in (A) and (C), respectively, showing rescued mutant (top) and wild-type JCVI-syn1.0 (bottom) populations.