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. 2014 Jul 23;6:162. doi: 10.3389/fnagi.2014.00162

Figure 2.

Figure 2

Cotinine reduces Aβ plaques in the hippocampus and entorhinal cortex of Tg6799 mice. Transgenic (Tg) mice at 4.5–5 months of age (n = 8 per group) were treated daily for 2 months with vehicle (Veh) or Cotinine (5 mg/kg; Cot 5), euthanized and Aβ immunoreactivity (IR) in the hippocampus and entorhinal cortex was measured by immunohistochemistry (IHC; A). Tg mice treated with cotinine (Tg + Cot 5) showed less Aβ plaques in the hippocampus and entorhinal cortex compared to vehicle-treated Tg mice (Tg + Veh; B). The photomicrographs to the left are representative views of Aβ plaques in the hippocampus (Upper) and entorhinal cortex (Lower) of brain sections of Tg mice stained with the Aβ-specific antibody 4G8 (Scale bar = 50 μm). The graphs to the right represent the percentage of Aβ plaque burden in these brain regions. In the hippocampus, a 47% reduction (t = 10.03; ***p < 0.001; C) and in the entorhinal cortex, a 45% reduction (t = 16.47; ***p < 0.001; D) was found. Tx, treatment.