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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Immunity. 2014 Jun 12;40(6):924–935. doi: 10.1016/j.immuni.2014.05.006

Figure 6. Atg5 and Atg3, but not Atg14L, are required for the localization of LC3 and Irga6 on the PVM of T. gondii.

Figure 6

Representative images (left) and quantitation (right) of immunofluorescence for T. gondii, LC3, and Irga6 in (A) Atg5flox/flox+/−LysMcre and (B) Atg14Lflox/flox+/−LysMcre BMDMs and (C) Atg3 WT and Atg3−/− MEFs at 2 hpi (MOI=1) of T. gondii infection +/− 24 hr pre-treatment of 100 U/ml of IFNγ. At least 100 cells infected T. gondii were analyzed for quantitation. Statistical analysis by 1-ANOVA with Tukey post test. n.s.: not significant (p>0.05), **: p<0.01, ***: p<0.001, ****: p<0.0001. Combined data as average ± SEM.