Table 2. Thermodynamic parameters of NADP binding to MATβ isoforms.
NADP → MATβV2 | NADP → MATβV1 | NADP → MAT(α2)4(βV2)2 | |
---|---|---|---|
[] | 500 µM → 30 µM | 500 µM → 29.8 µM | 155 µM→ 10.22 µM |
K a (M −1) | (1.12 ± 0.13) × 105 | (1.17 ± 0.22) × 105 | |
K d (M) | (8.93 ± 1.07) × 10−6 | (8.55 ± 1.6) × 10−6 | |
ΔH | 2784 ±117.4 | 1866 ± 127.8 | |
N | 0.85 ± 0.026 | 1.23 ± 0.060 |