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. 2014 Jul 23;5:344. doi: 10.3389/fimmu.2014.00344

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Copyright © 2014 Khan, Curbishley, Chen, Thomas, Pircher, Mavilio, Steven, Eberl and Moser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Negaitve effect of CD25⁺ cell depletion on expansion of γδT cells from melanoma patients. PBMC from healthy individuals (n = 10) and melanoma patients (n = 9) were depleted (CD25-neg) or not (PBMC) of Treg cells prior to stimulation with zoledronate plus IL-2/IL-15. Expansion fold is the ratio between the number of γδT cells in day 14 γδT cultures and the input γδT cells, i.e., the number of γδT cells present in PBMC at the beginning of cell culture.