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. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: J Hypertens. 2012 May;30(5):917–925. doi: 10.1097/HJH.0b013e3283525124

Figure 5.

Figure 5

Chromatin immunoprecipitation (ChIP)/Re-ChIP assays for recruitment and association of APE1 with HDAC1 corepressor complex on renin enhancer. (a) Effect of thapsigargin on APE1/HDAC1/HDAC2/mSin3A complex recruitment on the renin enhancer. α-APE1 or control immunoglobulin G (IgG) immunoprecipitates (IP) from crosslinked chromatin of thapsigargin-treated or control cells was eluted for second IP with α-HDAC1, α-HDAC2 or α-mSin3A antibody. ChIP analysis as in Fig. 3 was performed as described in methods. (b) Re-ChIP assay shows significant association of WT over NΔ33 APE1 with this corepressor complex. (c) Requirement of APE1 for corepressor complex binding to renin enhancer as shown by ChIP analysis. *, P<0.05; **, P<0.005.