Skip to main content
. 2014 Jul 1;53(28):4510–4518. doi: 10.1021/bi500610r

Figure 3.

Figure 3

Chemically modified siRNAs are allele-selective inhibitors of ATN-1 expression. Gels are Western analyses of ATN-1 levels. Graphs are based on triplicate determinations. CM is a control duplex RNA that is not complementary to ATN-1 mRNA. (A) Sequences, IC50 values, and selectivities of chemically modified duplex RNAs used for panels B–D. The bases shown in bold italics are mismatched relative to the CAG repeat region. Lowercase denotes 2′-O-methyl base substitutions. (B) Western analysis showing inhibition of ATN-1 protein expression when RNAs CMOD1, -2, -12, -13, -14, -22, and -23 and CM are added at a concentration of 3 nM. (C and D) Dose–response curves related to IC50 value determination for duplexes for CMOD12 and CMOD13, respectively.