Figure 4.

Steady-state kinetic analysis of the thiosulfate:glutathione sulfurtransferase reaction catalyzed by recombinant human TSTD1. Reactions were conducted in 50 mM Tris-acetate buffer (pH 9.0) at 37 °C. (A) Data obtained with 0.63, 1.25, 2.5, 5, and 15 mM glutathione are shown by the black, red, green, blue, and magenta circles, respectively. (B) Data obtained with 5, 10, 20, and 55 mM thiosulfate are shown by the black, red, green, and blue circles, respectively. The solid lines in panels A and B were obtained by fitting eq 2 to the data.