Table 1. Potential Candidate Genes for Yeast Thiosulfate Sulfurtransferasea.
gene |
|||||
---|---|---|---|---|---|
function | name | location | molecular weight (Da) | isoelectric point (pI) | no. of cysteine residues |
thiosulfate sulfurtransferaseb | unknown | unknown | ∼17000 | 5.1 | 1 |
unknown | RDL1 | YOR285W | 15413 | 5.91 | 1 |
unknown | RDL2 | YOR286W | 16697 | 9.65 | 1 |
thiolation tRNA | TUM1 | YOR251C | 34219 | 5.71 | 4 |
thiolation tRNA | UBA4 | YHR111W | 49361 | 6.12 | 13 |
phosphatase | YCH1 | YGR203W | 17248 | 7.01 | 2 |
thiolation tRNA, iron–sulfur cluster biogenesis | NFS1 | YCL017C | 54467 | 8.35 | 6 |
lipoic acid biosynthesis | LIP5 | YOR196C | 46247 | 9.55 | 10 |
biotin biosynthesis | BIO2 | YGR286C | 41884 | 8.76 | 10 |
thiolation tRNA | SLM3 | YDL033C | 47049 | 8.72 | 6 |
mitochondrial inner membrane transporterc | OAC1 | YKL120W | 35153 | 10.37 | 3 |
Unless otherwise noted, genes were retrieved by searching the S. cerevisiae genome database (http://www.yeastgenome.org) for entries that contained the term “sulfurtransferase” in the gene annotation or description.
Data reported by Uhteg and Westley40 for the enzyme isolated from S. cerevisiae.
Gene retrieved using “thiosulfate” as the search term.