Figure 10.

One-dimensional ¹H spectra of cyanomet rTHB1s at 298 K in a 10% ²H₂O/90% ¹H₂O mixture: (A) wild type at pH 7.5, (B) Y29F variant at pH 7.4, and (C) K53A variant at pH 7.7. Select assignments are indicated on panels A and B as listed in Table 2 and Tables S4–S6 of the Supporting Information. Blue labels refer to the major heme orientational isomer, red labels to the minor isomer, and green labels to Phe29 within the Y29F variant.