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. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: J Neuroimmunol. 2012 Oct 17;254(0):83–90. doi: 10.1016/j.jneuroim.2012.09.012

Fig. 2.

Fig. 2

Adjudin reduces LPS-induced cytokine production in BV2 microglia. Cells were pretreated with Adjudin for 1 h and then incubated with LPS. (A) After 24 h incubation, IL-6 release in supernatants was measured by ELISA. Data were normalized against the protein of the samples. (B–D) After 6 h incubation, TNF-α (B), IL-1β (C) and IL-6 (D) mRNA expression was evaluated by qPCR. Data were normalized by GAPDH. Bars represent the mean±SEM from four independent experiments. *P<0.05; **P<0.01; ***P<0.001, compared with the LPS alone treatment.