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. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: Sci Signal. 2013 Apr 2;6(269):ra22. doi: 10.1126/scisignal.2003405

Fig. 2.

Fig. 2

IPMK augments the transcriptional activity of p53. (A) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis (left) and quantification (right) of PUMA, Bax, and p21 mRNAs in U2OS cells transfected with plasmids encoding myc or mycIPMK and treated overnight with 10 μM etoposide. Data are means ± SEM from three experiments. ***P < 0.001, Student’s t test. (B) Western blotting analysis of PUMA, Bax, and p21 proteins in HCT116 cells transfected and treated as in (A). Data are means ± SEM from three experiments. *P < 0.05, **P < 0.01, Student’s t test. (C) Western blotting analysis of PUMA, Bax, and p21 proteins in U2OS cells transfected and treated as in (A). Data are means ± SEM from three experiments. *P < 0.05, **P < 0.01, Student’s t test. (D) qRT-PCR analysis (left) and quantification (right) of PUMA, Bax, and p21 mRNAs in fl/fl or Δ/Δ MEFs treated overnight with 20 μM etoposide. Data are means ± SEM from three experiments. ***P < 0.001, n = 3, mean ± SEM, Student’s t test.