Fig. 7.

Adjudin reduced lung and prostate carcinoma cells growth in vivo. A549 cells (A and B) and PC3 cells (C and D) were inoculated subcutaneously in the adult nude mice respectively. After 2 weeks when the tumors reached ~6 µM in diameter, Adjudin (dissolved in DMSO and corn oil) and vehicle (only with DMSO and corn oil) were administered every three days for six times in A549 (n = 12 for each group) and every other day for five times in PC3 (n = 12 for each group). Tumor growth was measured every 2 or 3 days after treatment and calculated using the formula V= 1/2 × LW², where L and W are the length and width of the tumor mass. The relative tumor volume was obtained by normalization to the initial tumor volume before Adjudin or vehicle treatment. Statistically significant differences on tumor volumes between the Adjudin and vehicle treatment groups (p < 0.0001 in A549, panel B; and ~0.006 in PC3, panel D) are determined by one-way ANOVA to be followed by Tukey’s Honest Significant Test. Representative images of subcutaneously grown tumors in nude mice after treatment of Adjudin or vehicle on Day 33 in A549 (A) and Day 25 in PC3(C) are also shown.