Figure 6.

Autophagy is engaged in KRN arthritis. Normal and day-9 arthritic paws were stained for LC3 by immunohistochemistry (IHC, A) or immunofluorescence (B). Autophagosome formation can be seen as punctated LC3 staining in arthritic paws (see higher magnification insets in B). Scale bar = 100 μm. (C) The percentage (%) of cells with punctated LC3 staining was significantly increased in arthritic paws (n = 3 mice per condition), ***p < 0.001 compared with normal paws. (D) LC3-I/LC3-II expression in paw lysates by immunoblotting. Actin serves as control for protein loading.