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. 2014 Jul 10;9:16. doi: 10.1186/1749-8104-9-16

Figure 10.

Figure 10

Anterograde labeling of retinal terminals in developing subnuclei of mouse visual thalamus. A. Retinal projections in P5 and P14 wild-type mice were labeled by intraocular injection of fluorescently conjugated cholera toxin subunit B (CTB). Left eyes were injected with Alexa Fluor 555 CTB (magenta) and right eyes were injected with Alexa Fluor 488 CTB (green). LGN from right hemispheres are shown. ‘Contra’ denotes projections originating from the contralateral retina and ‘ipsi’ denotes projections originating from the ipsilateral retina. Outlines of dorsal lateral geniculate nucleus (dLGN) and the external division of ventral lateral geniculate nucleus (vLGN) are depicted with white dots. d, dLGN; ve, external division of vLGN; i, IGL. B. High magnification images of CTB-labeled retinal terminals in dLGN and vLGN at P5 and P14. C. CTB-labeled retinal terminal areas were quantified in single optical sections of dLGN and vLGN at P5 and P14. Retinal terminal sizes in vLGN and dLGN were not statistically different from each other at P5 but statistically different at P14 (P <0.0001 by the Tukey Kramer test for differences between means). All images are maximum projection confocal images. Scale bar in A = 200 μm and in B = 15 μm.