Table 1.
Antibodies used in these studies
| Antigen | Isotype | Description of immunogen | Source/Catalog number | References | Dilution for immunohistochemistry |
|---|---|---|---|---|---|
| Glutamate Decarboxylase 67 (Gad 67) |
Mouse IgG2a |
Recombinant Gad67 |
Millipore #MAB5406 |
[67-69] |
1:500 |
| Glutamate Decarboxylase 65 (Gad 65) |
Rabbit IgG |
Synthetic peptide containing amino acids 572 to 585 of rat Gad65 |
Millipore #AB1511 |
[69,70] |
1:500 |
| Vesicular Glutamate Transporter 1 (VGluT1) |
Rabbit IgG |
Recombinant fusion protein consisting of amino acids 456 to 560 of rat VGlut2 |
Synaptic Systems #135303 |
[71,72] |
1:500 |
| Vesicular Glutamate Transporter 2 (VGluT2) |
Rabbit IgG |
Recombinant fusion protein consisting of amino acids 510 to 582 of rat VGlut2 |
Synaptic systems #135403 |
[72,73] |
1:500 |
| Vesicular GABA Transporter (VGAT) |
Rabbit IgG |
Synthetic peptide containing amino acids 510 to 525 of rat GABA |
Synaptic systems #131103 |
[74,75] |
1:500 |
| Vesicular Acetyl-choline Transporter (VAChT) | Guinea Pig IgG | Fusion protein consisting of amino acids 475 to 530 of rat VAChT | Synaptic systems #139105 | [76] | 1:250 |