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. Author manuscript; available in PMC: 2014 Nov 30.
Published in final edited form as: Science. 2014 May 1;344(6187):1042–1047. doi: 10.1126/science.1251871

Fig. 1. Bacterial NET-seq provides a genome-wide view of transcription dynamics.

Fig. 1

(A) Nascent RNA is isolated from bacteria and converted to a DNA library sequenced with deep coverage. Reads are aligned to the reference genome and mapped according to their 3′ end, which corresponds to the RNAP active site. (B) An example of RNAP density in the his leader region (hisL) shows a peak at a single site which matches the previously mapped regulatory pause position (underlined sequence). (C) Biological replicates along the ribosomal L10 protein subunit (rplJ). (D) Histogram of pause frequency for highly transcribed genes (n = 1984, gene average >1 read/bp) within the protein coding sequence.