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Osteoblast differentiation in gels. MC3T3 cells were cultured in gel beads with different RGD island spacings (○ no RGD; □, 36 nm; △, 78 nm), while maintaining a constant bulk ligand density ([RGD] = 6.25 μg/mg polymer) in the latter two conditions, and the secretion level of osteocalcin was quantified and normalized by the amount of DNA.
