Table 1.
bulk RGD densitya (μg/mg alginate) | number of peptides per alginate chain | dilution factor | idealized RGD island spacing (nm) |
---|---|---|---|
0.125 | 1 | 50 | 134 |
2 | 100 | 168 | |
1.25 | 1 | 5 | 62 |
2 | 10 | 78 | |
6.25 | 1 | 1 | 36 |
4 | 4 | 58 | |
10 | 10 | 78 | |
12.5 | 2 | 1 | 36 |
The mole ratio of carboxylate/sulfo-NHS/EDC in the reaction was kept constant at 40:1:2, and the amount of RGD peptides was varied. The reaction time was 24 h.