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Binding of fluorescent pentamidine analogs to CTG*CAG repeat hairpin DNA. a) Structure of fluorescent pentamidine analogs prop-BAPPA (n=3), pent-BAPPA (n=5), and hept-BAPHA (n=7). b-d) Representative CTG*CAG titrations into 0.5 μM b) prop-BAPPA, c) pent-BAPPA, and d) hept-BAPHA. e) Maximum emission of each DNA concentration curve. Data were zeroed and fit with rectangular hyperbola in order to obtain an apparent Kd. Error bars are standard deviation of three observations.
