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. 2014 Jul 23;9(7):e101280. doi: 10.1371/journal.pone.0101280

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© 2014 Song et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Western blot analyses of RbmC levels in the culture supernatants of the wild type V. cholerae strain A1552 and vrrA mutant derivatives: (A) Detection of RbmC (upper panel) in the supernatants from DNY7 (ΔvrrA) carrying different plasmids expressing either the wild-type VrrA (from plasmid pTS2) or mutant variants VrrA^M1 to VrrA^M6 are expressed from plasmids pTS2-M1 to pTS2-M6. A SDS-PAGE Coomassie blue stained gel is shown as the sample loading control (lower panel). (B) Western blot analysis of the RbmC levels in supernatants isolated from DNY7 (ΔvrrA) and DNY189 (ΔvrrA rbmC*) carrying different plasmids (upper panel). A SDS-PAGE gel stained with Coomassie blue is shown as loading (lower panel). Protein marker sizes are given to the left in kDa.