TABLE II.
Subtomogram alignment error in terms of the difference in the Euclidean distance between determined and true subtomogram translations. Shown are the medians and median absolute deviations of all 100 subtomogram alignments.
RCS | ||||
---|---|---|---|---|
SNR | ∞ | 1 | 0.5 | 0.1 |
Tilt | ||||
±90° | 0.035 ± 0.023 | 0.16 ± 0.12 | 0.19 ± 0.12 | 0.96 ± 0.66 |
±80° | 0.045 ± 0.029 | 0.24 ± 0.2 | 0.21 ± 0.15 | 1.3 ± 0.89 |
±70° | 0.078 ± 0.037 | 0.25 ± 0.17 | 0.3 ± 0.18 | 1.3 ± 0.74 |
±60° | 0.068 ± 0.036 | 0.19 ± 0.12 | 0.43 ± 0.3 | 2.2 ± 1.3 |
±50° | 0.14 ± 0.078 | 0.26 ± 0.17 | 0.65 ± 0.51 | 2.3 ± 1.3 |
±40° | 0.15 ± 0.092 | 0.74 ± 0.64 | 1.7 ± 1.3 | 3.2 ± 1.6 |
FCS | ||||
∞ | 1 | 0.5 | 0.1 | |
±90° | 0.047 ± 0.023 | 0.12 ± 0.081 | 0.11 ± 0.053 | 0.49 ± 0.31 |
±80° | 0.053 ± 0.03 | 0.15 ± 0.1 | 0.18 ± 0.1 | 0.85 ± 0.66 |
±70° | 0.11 ± 0.057 | 0.13 ± 0.074 | 0.21 ± 0.1 | 0.95 ± 0.58 |
±60° | 0.11 ± 0.061 | 0.2 ± 0.094 | 0.3 ± 0.15 | 1.6 ± 1.2 |
±50° | 0.19 ± 0.1 | 0.28 ± 0.16 | 0.44 ± 0.26 | 1.8 ± 1.2 |
±40° | 0.61 ± 0.54 | 3.3 ± 2.7 | 4.3 ± 2.6 | 6.2 ± 3 |