Table 1. Generation of HAdv-7-neutralizing monoclonal antibodies.

MAb	Isotype	Ascites titera	IgG concentration (mg/ml)b	Neutralization titer against virusesc
				HAdv-7	Ad3EGFP
1C7	IgG1	50,000	3.36	512	<16
3G5	IgG2a	1,000,000	16.94	2048	<16
1D9	IgG1	50,000	17.71	1024	<16
6F3	IgG1	1,000,000	19.22	2048	<16
3D7d	IgG2a	100,000	7.13	<16	1024

^aThe ascites titer against rAdMHE3 is expressed as the reciprocal of the ascites dilution and was determined by indirect ELISA.

^bEach ascites (1 ml) was purified by octanoic acid–ammonium sulfate precipitation to a final volume of 150 µl and the IgG concentration was determined spectrophotometrically using a conversion factor of 1.4 mg/ml per absorbance unit at 260 nm [22].

^cNeutralization titer is expressed as the reciprocal of the ascites dilution and was determined as the highest dilution of ascites that protected HEp-2 cell monolayers from a visually observable CPE.

^d3D7 was one of the 22 MAbs isolated from mice vaccinated with rAdMHE3 virions. We used 3D7 here as a control.