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. 2014 Jul 23;9(7):e103432. doi: 10.1371/journal.pone.0103432

Figure 4. Annexin A2 promotes glioma IL-1 synthesis and release.

Figure 4

The role of Annexin A2 in IL-1 production was examined by ELISA following transfection with A2-specific or control siRNA. (A) Western blot showing robust amounts of A2 protein expression in U87 and U251 compared to normal human astrocytes. (B) Western blot showing decreased A2 expression in U251 cells transfected with A2-specific siRNA vs. control siRNA. (C, D) The amount of IL-1β protein (intracellular and secreted) was determined by ELISA in U251 and U87 cultures following A2 knockdown. Data are mean ± SD (n = 3) *** p<0.001, ** p<0.01 (si Ctr vs. si A2), t-test.