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Representative immunohistochemistry images of PIK3CA expression across multiple tumor grades. PIK3CA protein was localized on the cytoplasm of tumor cells. Tumors were scored on a scale from grade 0 to 3+. (A) grade 0, 100×; (B) grade 0, 200×; (C) grade 1+, 100×; (D) grade 1+, 200×; (E) grade 2+, 100×; (F) grade 2+, 200×; (G) grade 3+, 100×; (H) grade 3+, 200×; (I) normal mucosa, 100×; and (J) normal mucosa, 200×.
