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. 2014 Jul 23;9(7):e102948. doi: 10.1371/journal.pone.0102948

Figure 1. PGE2 promotes mast cell death during cytokine deprivation.

Figure 1

A. The percentage of 7-AAD stained mast cells after culture in complete medium with 1×10−6 M PGE2, in depletion medium without cytokines and in depletion medium with 1×10−6M PGE2. Data are from 8 independent experiments using 2 cultures of WT BMMC. B. The percentage of 7AAD stained cells during cytokine deprivation for 16 h carried out in the presence of various PGE2 concentrations. Data are from 8 independent experiments using 1 culture of WT BMMC. C. 7-AAD positive BMMC pretreated with 1×10−6 M PGE2 in complete medium for various times. Cells were depleted from cytokines for 16 h without further presence of PGE2. Data are from 4 independent experiments using 1 culture of WT BMMC. CTRL indicates mast cells cultured in complete medium during the experiment. ANOVA was used in B and C to evaluate statistical significance. Statistical significance: * = P<0.05, ** = P<0.01.