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. 2014 Jul 23;9(7):e102948. doi: 10.1371/journal.pone.0102948

Figure 6. PGE2-stimulated MAPK activation is dependent on calcium and calmodulin dependent kinase II.

Figure 6

A. Erk1/2 phosphorylation is inhibited by BAPTA. Cells were pretreated with various concentration of calcium inhibitor BAPTA before activation with 1×10−6 M PGE2, n = 3 independent experimetns of 1 culture of BMMC. B. Calmodulin dependent kinase II specific inhibitor KN-93 inhibited PGE2 increased apoptosis. BMMC were pretreated with various concentration of KN-93 for 10 min followed by activation with by 1×10−6 M PGE2. Dead cells stained with 7-AAD, n = 6 independent experiments of 3 cultures of BMMC. C. KN-93 at various concentrations inhibited Erk1/2 phosphorylation induced by 1×10−6 M PGE2, n = 4 independent experiments of 1 cultures of BMMC. BMMC cultured in complete medium (CTRL), medium without cytokines (no cyt.) and medium without cytokines in the presence 1×10−6 M PGE2 (no cyt. + PGE2). Statistical significance using ANOVA: * = P<0.05, ** = P<0.01, *** = P<0.001.