Skip to main content
. Author manuscript; available in PMC: 2014 Jul 23.
Published in final edited form as: Nat Med. 2010 Jul 4;16(7):774–780. doi: 10.1038/nm.2175

Figure 4.

Figure 4

HST3 repression and nicotinamide are cytotoxic to C. albicans strains that express RTT109. (a) Immunoblots of H3K56ac in wild-type (SN152) and rtt109Δ/Δ cells treated with 100 mM nicotinamide (+NAM). (b) Colony formation assays performed in the absence (YPD) or presence of either nicotinic acid (NA) or nicotinamide (NAM). (c) The growth of each strain (monitored by OD620 in a microtiter plate assay) at a given NAM concentration, as plotted as a percentage of its growth in the absence of NAM. (d) The effect of NAM on wild-type SN152 cells (50 mM NAM at 30 °C for 24 h), as monitored by DIC and fluorescence microscopy to detect either DNA (DAPI) or cell walls and septa (CFW).