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. Author manuscript; available in PMC: 2015 Aug 12.
Published in final edited form as: J Mol Biol. 2014 Jun 12;426(16):2901–2917. doi: 10.1016/j.jmb.2014.06.003

FIGURE 5.

FIGURE 5

Stopped-flow kinetics of dTTP incorporation into S-1 DNA catalyzed by Klentaq1 at 20 °C. A pre-incubated solution of Klentaq1 mutant (600 nM) and DNA substrate S-1 (100 nM) was rapidly mixed with dTTP (0.5 mM) and fluorescence was monitored using a stopped-flow apparatus. Donor (black) and acceptor (gray) traces are shown for (A) the intervening (K346C), (B) Finger (V649C), and (C) Palm (A814C).