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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Immunol. 2014 Jul 2;193(3):1364–1372. doi: 10.4049/jimmunol.1303322

Figure 6.

Figure 6

(A) DHX15 is required to induce MAVS aggregation. Immunoblot (IB) of MAVS by SDD-AGE and SDS-PAGE in crude mitochondrial extracts from DHX15-knockdown D2SC after 6 hr stimulation with 5.0 μg/ml of poly I:C delivered by Lipofectamine™ 2000 or without stimulation. Scrambled shRNA treated D2SC cells served as a control. (B) DHX15 and MAVS are required for activating the NF-κB, IRF3 and MAPK signal transduction pathways upon reovirus infection. Immunoblot of indicated proteins from lysates of D2SC with indicated shRNA after infection with reovirus at a multiplicity of infection of 10. GAPDH served as loading control.