Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Bone Miner Res. 2014 Aug;29(8):1900–1913. doi: 10.1002/jbmr.2196

Figure 2. IGF-IR in the osteoblasts or osteoclasts is required for the expression of ephrin B2 and EphB4 in osteoblasts and the expression of ephrin B2 in osteoclasts.

Figure 2

A: Expression of ephrin B2 and EphB4 mRNA levels in bones (marrow flushed out) of osteoblast-specific IGF-IR KO mice (OBIGF-IR KO) and control littermates treated with PTH (open bars) or vehicle (solid bars) for 2 weeks was determined by Q-PCR. n = 4 in vehicle-treated control and OBIGF-IR KO mice and n =5 in PTH-treated control and OBIGF-IR KO mice. B–C: BMSCs (B, OB progenitors) or spleen cells (C, OCL precursors) from the floxed IGF-IR mice were treated with PBS (vehicle control), or infected with empty viruses (DNR, hatched bar, virus control) or adenoviruses carrying a functional cre-recombinase (Adv-cre) to delete IGF-IR in these cells. BMSCs were further treated by PTH (100 ng/ml, open bars) or vehicle (solid bars) for 2 hrs. D: BMSC from the wild-type mice (WT) were treated with PBS (solid bar) or Adv-cre (open bar) to test the effect of Adv-cre infection on WT BMSCs. mRNA levels of ephrin B2, EphB4 and IGF-IR were determined by Q-PCR. In A–B, a: p < 0.05 vs. vehicle treated control; b: p < 0.05 vs. PTH treated controls; in C, a: p < 0.05 vs. PBS control. n = 4 wells in each group. Error bars enclose mean ± SD.