Skip to main content
. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: J Bone Miner Res. 2014 Aug;29(8):1900–1913. doi: 10.1002/jbmr.2196

Figure 5. Ephrin B2 in osteoclasts promotes osteoblast and osteoclast differentiation.

Figure 5

Osteoblasts were co-cultured with spleen cells (osteoclast precursors) from floxed ephrinB2 mice infected with control (control), empty viruses (Ad-DNR, virus control) or adenoviruses carrying a functional cre-recombinase (Adv-cre) prior to co-culture. These co-cultures were further treated by vehicle or IGF-I. A: TRAP staining. The number of osteoclasts (arrows) per low magnification field (LMF) formed in the cultures was counted and shown under the corresponding pictures. The mRNA levels of ephrin B1 and ephrin B2 receptors (B) and osteoblast (osx) and osteoclast (NFATc1) differentiation markers (C) in the co-cultures were determined by Q-PCR. V: vehicle treated co-cultures, IGF-I: IGF-I treated co-cultures. Results are expressed as mean ± SD. a: p < 0.05 vs. vehicle treated control; b: p < .0.5 vs. vehicle treated Ad-Cre. n = 4 wells in each group