Skip to main content
. Author manuscript; available in PMC: 2015 Aug 12.
Published in final edited form as: J Mol Biol. 2014 Jun 6;426(16):2928–2941. doi: 10.1016/j.jmb.2014.05.028

Figure 4.

Figure 4

Sus1p promotes the PIC formation at the ADH1 and PHO84 core promoters. (A and B) Sus1p is required for recruitment of TBP and RNA polymerase II to the ADH1 and PHO84 core promoters. Yeast strains were grown in YPD (yeast extract containing peptone plus 2% dextrose) up to an OD600 of 1.0 prior to crosslinking. Immunoprecipitation was carried as in Figures 2A and 2B. The ChIP signal of the wild type strain was set to 100, and the ChIP signal of the Δsus1 strain was normalized with respect to 100. The normalized ChIP signal (represented as normalized occupancy) is plotted in the form of a histogram. (C) Transcription. Total cellular RNA was prepared from the wild type and Δsus1 strains, and mRNA levels from the ADH1, PHO84 and RPS5 genes were quantitated by primer extension analysis. Yeast cells were grown as in panels A and B. (D) Growth analysis. Both wild type and Δsus1 strains were initially grown in YPD up to an OD600 of 0.1 at 30 °C. Subsequently, OD600 was measured for next 5 hr in YPD, and plotted as a function of time. (E) Growth analysis of the Δsus1 and wild type strains in the solid SC-uracil medium containing 2% dextrose with or without 6-AU (100 μg/ml) at 30 °C.