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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Cardiovasc Intervent Radiol. 2014 Jan 23;37(5):1343–1351. doi: 10.1007/s00270-013-0828-3

Fig. 1.

Fig. 1

Effect of heat stress temperature on N1S1 and AS30D HCC cell viability. N1S1 and AS30D cells heat stressed at indicated temperatures from 37 to 60 °C for 10 min were assessed for viability with WST-1 assay at 48 h after heat stress. Data were normalized to 37 °C control and presented as mean ± SEM of 3 independent experiments. Nonlinear regression curve fitting was used to calculate heat stress IT50. IT50 was defined as temperature that induces 50 %reduction in cell viability relative to 37 °C control for 10-min exposure time