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Methods for generating 3D ROI (region of interest) used in micro-CT analysis of alveolar bone. Two-dimensional contours were drawn manually every five data planes on the axial view, from the roof of the furcation to the root apex. Root surface area was excluded from the bone surface area on every measured plane. A 3D ROI was created after all the 2D contours were drawn. Alveolar bone volume was calculated as total ROI minus root volume.
