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. Author manuscript; available in PMC: 2015 Jul 21.
Published in final edited form as: Curr Biol. 2014 Jul 3;24(14):1573–1583. doi: 10.1016/j.cub.2014.06.004

Figure 1. Proteasome inhibitor cause induction of all 26S proteasome subunits, p97, and its cofactors in ERAD (See also Fig. S1).

Figure 1

(A) Treatment of neuroblastoma SH-SY5Y cells with BTZ (10nM, 16h) induced mRNAs for all 26S subunits, measured by RT-PCR. (p<0.05 for all changes) (B) and increased the levels of Rpt5 and 20S subunits measured by Western blot (WB). Biological duplicates were assayed, and levels of Ub conjugates were measured to prove proteasome inhibition. (C) Treatment of SH-SY5Y cells with BTZ (10nM, 16h) also induced mRNAs for p97 and its cofactors, Npl4, Ufd1, and p47, and (D) increased content of p97 protein. (*: p<0.05). Error bars represent standard deviation (Error bar=SD).