. 2014 Mar 7;76(6):877–885. doi: 10.1292/jvms.14-0028

Table 2. Species- and genus-specific primers for the quantification of ruminal bacteria using real-time PCR assay.

Bacteria	Primer	Sequence (5′- 3′)	Source
Lactobacillus plantarum	Sg-Lpla-F	CTCTGGTATTGATTGGTGCTTGCAT	[22]
Lactobacillus plantarum	Sg-Lpla-R	GTTCGCCACTCACTCAAATGTAAA	[22]
Enterococcus spp.	g-Bfra-F	CCCTTATTGTTAGTTGCCATCATT	[31]
Enterococcus spp.	g-Bfra-R	ACTCGGTTGTACTTCCCATTGT	[31]
Clostridium butyricum	209F25	AGTGATTGTCAGTAGTAGACGAGCG	[26]
Clostridium butyricum	R221	CATGCGCCCTTTGTAGC	[26]
Clostridium coccoides	g-Coc-F	AAATGACGGTACCTGACTAA	[23]
Clostridium coccoides	g-Coc-R	CTTTGAGTTTCATTCTTGCGAA	[23]
Megasphaera elsdenii	MegEls1F	GACCGAAACTGCGATGCTAGA	[13]
Megasphaera elsdenii	MegEls1R	CGCCTCAGCGTCAGTTGTC	[13]
Selenomonas ruminantium	SelRum1F	GGCGGGAAGGCAAGTCAGTC	[13]
Selenomonas ruminantium	SelRum1R	CCTCTCCTGCACTCAAGAAAGACAG	[13]