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. 2014 Jul 24;4:5814. doi: 10.1038/srep05814

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(A) Smad2/3 phosphorylation was determined by western blotting of NRK52E cells stimulated by TGF-β₁ (5 ng/ml) in a time course experiment. (B) Smad2/3 phosphorylation was determined by western blotting of NRK52E cells cultured with different concentration of KS370G (0.1 to 3 μM) for 30 minutes under TGF-β₁ stimulation. (C) Quantitative results presented as mean ± SEM of the signal's optical density (n = 5). *P < 0.05 compared with control group. #P < 0.05 compared with TGF-β₁ (5 ng/ml) groups.