Table 1.
Nucleotide Sequences of the Forward and Reverse Primers Used for qPCR
| Gene | Accession number | Forward primer | Reverse primer |
|---|---|---|---|
| elf1α | AY422992 | CTGGAGGCCAGCTCAAACAT | TCAAGAAGAGTAGTACCGCTAGCATTAC |
| rpl13 | NM_212784 | TCTGGAGGACTGTAAGAGGTATGC | AGACGCACAATCTTGAGAGCAG |
| bdnf | NM_131595 | AGAGCGGACGAATATCGCAG | GTTGGAACTTTACTGTCCAGTCG |
| nr3c1(alpha) | EF436284.1 | ACTCCATGCACGACTTGGTG | GCATTTCGGGAAACTCCACG |
| nr3c1(beta) | EF436285.1 | GATGAACTACGAATGTCTTA | GCAACAGACAGCCAGACAGCTCACT |
| nr3c2 | NM_001100403 | CTTCCAGGTTTCCGCAGTCTAC | GGAGGAGAGACACATCCAGGAAT |
| cart4 | NM_001082932 | GCTGAGGCACTCGATGAACT | GAAGAAAGTGTTGCAGGCGG |
| htr1ab | NM_001145766 | GGACATTAAAACGCGCTGCT | ATGCAAGTCTTGGGTTGAGACT |
Elongation factor 1-alpha (elf1α) and ribosomal protein L13 (rpl13) were used as housekeeping genes. Genes of interest were brain-derived neurotrophic factor (bdnf), serotonin receptor 1ab (htr1ab), cocaine- and amphetamine-regulated transcript (cart4), mineralocorticoid receptor (nr3c2), and the glucocorticoid receptor alpha [nr3c1(alpha)] and glucocorticoid receptor beta [nr3c1(beta)].