Skip to main content
. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Methods Mol Biol. 2014;1140:89–105. doi: 10.1007/978-1-4939-0354-2_7

Fig. 2.

Fig. 2

Purification of two His6-labeled proteins monitored by SDS-PAGE (Lonza PAGEr™ Gold 4–20 % gradient precast gel). Lanes 1 and 6: Soluble cell extracts. Lanes 2 and 7: Pooled peak fractions after IMAC-I. Lanes 3 and 8: Cleavage of His6- tag with TEV protease. Lanes 4 and 9: Pooled peak fractions after IMAC-II. Lane 5: Molecular weight standards (kDa). IDP0564 (gi|126699790; amino acid ABC transporter, substrate-binding protein [Clostridium difficile 630]) and IDP05558 (gi|16803797; hypothetical protein lmo1757 [Listeria monocytogenes EGD-e]) are targets from the Center of Structural Genomics of Infectious Diseases (CSGID)